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The effect of Chd7/8 knockdown on the DNA damage response in lung adenocarcinoma. A Pearson correlation analysis showing the correlation between CHD7/8 expression and RAD51 expression in LUAD; B RT–qPCR analysis of RAD51 upon CHD7 and CHD8 silencing; C , D Western blot analysis of Rad51 (C), γH2AX and p-chk1 (D) proteins upon depletion of CHD7 and CHD8; E , F Growth inhibition curves of cisplatin ( E ) and paclitaxel ( F ) in A549 and PC9 cells with CHD7 and CHD8 knockdown. The cell viability was assessed by the CellTiter-Glo assay. The IC50 values were determined from the sigmoidal dose–response curves using GraphPad <t>PRISM6</t> software
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The effect of Chd7/8 knockdown on the DNA damage response in lung adenocarcinoma. A Pearson correlation analysis showing the correlation between CHD7/8 expression and RAD51 expression in LUAD; B RT–qPCR analysis of RAD51 upon CHD7 and CHD8 silencing; C , D Western blot analysis of Rad51 (C), γH2AX and p-chk1 (D) proteins upon depletion of CHD7 and CHD8; E , F Growth inhibition curves of cisplatin ( E ) and paclitaxel ( F ) in A549 and PC9 cells with CHD7 and CHD8 knockdown. The cell viability was assessed by the CellTiter-Glo assay. The IC50 values were determined from the sigmoidal dose–response curves using GraphPad <t>PRISM6</t> software
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The effect of Chd7/8 knockdown on the DNA damage response in lung adenocarcinoma. A Pearson correlation analysis showing the correlation between CHD7/8 expression and RAD51 expression in LUAD; B RT–qPCR analysis of RAD51 upon CHD7 and CHD8 silencing; C , D Western blot analysis of Rad51 (C), γH2AX and p-chk1 (D) proteins upon depletion of CHD7 and CHD8; E , F Growth inhibition curves of cisplatin ( E ) and paclitaxel ( F ) in A549 and PC9 cells with CHD7 and CHD8 knockdown. The cell viability was assessed by the CellTiter-Glo assay. The IC50 values were determined from the sigmoidal dose–response curves using GraphPad PRISM6 software

Journal: Discover. Oncology

Article Title: Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer

doi: 10.1007/s12672-022-00489-y

Figure Lengend Snippet: The effect of Chd7/8 knockdown on the DNA damage response in lung adenocarcinoma. A Pearson correlation analysis showing the correlation between CHD7/8 expression and RAD51 expression in LUAD; B RT–qPCR analysis of RAD51 upon CHD7 and CHD8 silencing; C , D Western blot analysis of Rad51 (C), γH2AX and p-chk1 (D) proteins upon depletion of CHD7 and CHD8; E , F Growth inhibition curves of cisplatin ( E ) and paclitaxel ( F ) in A549 and PC9 cells with CHD7 and CHD8 knockdown. The cell viability was assessed by the CellTiter-Glo assay. The IC50 values were determined from the sigmoidal dose–response curves using GraphPad PRISM6 software

Article Snippet: The IC50 values were determined from the sigmoidal dose–response curves using GraphPad PRISM6 software

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Inhibition, Glo Assay, Software